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The endonuclease HaeIII recognizes ______________________ sequence.

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The endonuclease HaeIII recognizes ______________________ sequence.

(a) mono-nucleotide

(b) bi-nucleotide

(c) tri-nucleotide

(d) tetra-nucleotide

This question was addressed to me during an online interview.

The question is from Techniques in portion Techniques in Cell Biology of Cell Biology

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Right choice is (c) tri-nucleotide

Easy explanation: The endonuclease HaeIII and Sau3A, are tetranucleotide sequences used in the construction of genomic DNA libraries. After digestion of the nucleotides, the fragments are separated by gel electrophoresis.

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