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Which of the following can be used to clone DNA sequence of size larger than 25 kb?

(a) Yeast artificial chromosome

(b) SV40

(c) Plasmid

(d) Bacteriophage

The question was posed to me in an international level competition.

My question is from Techniques in division Techniques in Cell Biology of Cell Biology

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Right option is (a) Yeast artificial chromosome

The explanation is: For cloning DNA sequences of a size larger than 20 to 25 kb, the conventional vectors such as plasmids and bacteriophages can not be used. For the cloning of large DNA fragments, the yeast artificial chromosome (YAC) may be used.

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