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In gel-filtration chromatography, the proteins ______________________

(a) bind to the column

(b) diffuse through the column

(c) denature

(d) vaporize

This question was posed to me in an online quiz.

My question is based upon Protein Techniques in portion Techniques in Cell Biology of Cell Biology

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The correct option is (b) diffuse through the column

For explanation I would say: The gel used for this technique used small beads that are tightly packed in the column. This causes a small void to form between the beads. The proteins that are larger than the voids pass through or diffuse through the gel.

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