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Rho dependent termination mechanism doesn’t reguire____________

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Rho dependent termination mechanism doesn’t reguire____________

(a) ATP

(b) Stem loop structure

(c) G-C rich stem

(d) Sigma factor

I got this question in class test.

Origin of the question is Transcription in Prokaryotes : Termination topic in chapter Gene Expression : Transcription Prokaryotes of Cytogenetics

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The correct choice is (d) Sigma factor

The best explanation: Sigma factor plays no role in termination, rather it dissociates long before the RNA pol terminates. Rho however requires ATP for its rolling function and the stem loop structure formation. This stem loop structure is strengthened by G-C rich regions in the stem.

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