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Puromycin is a ribosomal antibiotic. It binds to ________

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Puromycin is a ribosomal antibiotic. It binds to ________

(a) Peptide exit channel

(b) 16S rRNA

(c) tRNA binding site

(d) A site of unassociated 16S rRNA

This question was addressed to me during an internship interview.

I'm obligated to ask this question of Ribosome topic in portion Gene Expression : Translation of Cytogenetics

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The correct option is (c) tRNA binding site

For explanation: Puromycinis a tRNA analogue that has an amide bond in place of ester bond. This is why when the ribosome transfers the peptide over this; it flees with the peptide and stops the translation.

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