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Which gene in a plasmid is indispensible for it to participate in conjugation?

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Which gene in a plasmid is indispensible for it to participate in conjugation?

(a) Pilli forming gene

(b) Origin of replication

(c) Sex determining gene

(d) Origin of transfer

I got this question in my homework.

The question is from Gene Mapping in Bacteria by Conjugation in portion Genomics : The Mapping and Sequencing of Genomes of Cytogenetics

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Correct choice is (c) Sex determining gene

The best I can explain: There is no particular sex determining gene in bacteria, their pseudo sex is determined by presence or absence f fertility factor. However pilli forming gene is necessary for the conjugation channel, ori of transfer and ori of replication also maintain a plasmid.

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