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Which of the following will not affect translation from the AUG involved?

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Which of the following will not affect translation from the AUG involved?

(a) A short hairpin near the cap

(b) A hairpin 52 at downstream of cap

(c) A long hairpin between cap and initiation codon

(d) Providing a purine at -3 and +4 position of a start codon

The question was asked in my homework.

My question is based upon Initiation in portion Gene Expression : Translation of Cytogenetics

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The correct answer is (b) A hairpin 52 at downstream of cap

To explain I would say: It has been seen experimentally that a hairpin 52 at further from the cap can’t help the AUG to be recognized as initiation site even when the AUG is within it. The other options here improve translation from the AUG involved.

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