Question

In case of chicken foot Holliday intermediate, the template strand for synthesis across lesion is___________

(a) The parent strand aligned along strand with lesion

(b) The parent strand aligned against lesion strand

(c) The daughter strand with lesion

(d) The daughter strand without lesion

This question was addressed to me at a job interview.

The above asked question is from Homologous DNA Recombination topic in chapter Genetic Recombination of Cytogenetics

Answer 1

Correct answer is (d) The daughter strand without lesion

For explanation I would say: In this model to bypass the lesion the daughter strand synthesized from the other parent strand is used as a template. This codes for the correct bases against the lesion and is made possible by fork regression.