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Artificial transformation in the laboratory is carried out by which of the chemical?

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Artificial transformation in the laboratory is carried out by which of the chemical?

(a) Sodium chloride

(b) Magnesium chloride

(c) Calcium chloride

(d) Potassium chloride

I have been asked this question during a job interview.

This intriguing question comes from Genetic Recombination topic in chapter Genetic Recombination of Drug Biotechnology

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Right choice is (c) Calcium chloride

Best explanation: In laboratory artificial transformation is done by treatment of the cells using calcium chloride. This technique also makes the membranes more permeable to the uptake DNA. We can use this technique with species which are naturally competent.

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