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What should be the minimum weight of DNA that is required for a successful transformation?

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What should be the minimum weight of DNA that is required for a successful transformation?

(a) 1000 Daltons

(b) 100,000 Daltons

(c) 300,000 daltons

(d) 8 million daltons

This question was posed to me in class test.

The doubt is from Genetic Recombination topic in section Genetic Recombination of Drug Biotechnology

1 Answer

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Correct answer is (c) 300,000 daltons

Easiest explanation: Molecular weights of DNA in the range of 300,000 to 8 million daltons have been shown to result in successful transformation. Various vectors have been manufacture including HAC, BAC, YAC which can transfer bigger pieces of genomes.

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