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In a chemostat operating under steady state, a bacterial culture can be grown at dilution rate higher than the maximum growth rate by _________

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In a chemostat operating under steady state, a bacterial culture can be grown at dilution rate higher than the maximum growth rate by _________

(a) Partial cell recycling

(b) Using sub optimal temperature

(c) pH cycling

(d) Substrate feed rate cycling

I have been asked this question by my college professor while I was bunking the class.

Asked question is from Antibiotics topic in chapter Antibiotics & Enzyme Immobilization of Drug Biotechnology

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The correct choice is (a) Partial cell recycling

To explain: D = F/V

Dilution rate is the rate with which substrate is added to a continuous system and products are removed from the system. When dilution rate is higher than maximum growth rate wash out condition occurs in which the cell concentrations progressively decreases. Only way of culturing biomass in the case of wash out is by partially recycling the cells, which were removed from the system.

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