Question

If the substrate contains ionic groups, what happens to the pH of the medium?

(a) The pH of medium increases

(b) The pH of medium decreases

(c) The pH of medium remains same

(d) The pH of medium affects the affinity of substrate to enzyme

I have been asked this question at a job interview.

The question is from Methods of Immobilization in section Antibiotics & Enzyme Immobilization of Drug Biotechnology

Answer 1

Right option is (d) The pH of medium affects the affinity of substrate to enzyme

Easy explanation: Some cases, the substrate may contain ionic groups, and the pH of medium affects the affinity of substrate to enzyme. This may lead to major changes in the binding of the enzyme to the substrate. This may damage some vital reactions in our body.