Question

Variation in pH of medium result in changes of ionic form of the active site.

(a) True

(b) False

This question was posed to me in class test.

My doubt is from Methods of Immobilization topic in chapter Antibiotics & Enzyme Immobilization of Drug Biotechnology

Answer 1

The correct answer is (a) True

Easy explanation: Variation in pH of medium result in changes of Ionic form of the active site and activity of enzyme, hence the reaction rate. Variation in pH of medium can also result in changes of the maximum reaction rate, Km stability of the enzyme. Enzyme are active only over small range of pH due to the active site functional group charges (ionic form) and the three dimensional shape of enzyme are pH-dependent.