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What is the detection technique of auxotrophs?

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What is the detection technique of auxotrophs?

(a) Spread plating

(b) Replica plating

(c) Streaking

(d) Pouring

The question was asked in a national level competition.

I need to ask this question from Mutation topic in portion Molecular Genetics of Life Sciences

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Correct option is (b) Replica plating

Easy explanation: Auxotrophs is a conditional lethal mutation in which mutants need essential metabolites for proper growth.

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