Question

Fragments of DNA formed after treatment with endonucleases are separated by the technique of _______

(a) polymerase chain reaction

(b) gene amplification

(c) blotting

(d) gel electrophoresis

This question was posed to me in quiz.

My enquiry is from Tools of Recombinant DNA Technology: Restriction Enzymes topic in chapter Biotechnology Principles and Processes of Biology – Class 12

Answer 1

The correct option is (d) gel electrophoresis

To explain I would say: DNA is a charged molecule. When fragmented, it can be separated by the technique called gel electrophoresis. It is a technique in which the molecules are separated based on the charge present on that molecule.