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What temperature is suitable for heat-shock treatment?

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in Biology – Class 12 by (271k points)
What temperature is suitable for heat-shock treatment?

(a) 100°C

(b) 0°C

(c) 42°C

(d) 95°C

I had been asked this question in an online interview.

Origin of the question is Tools of Recombinant DNA Technology: Competent Host in portion Biotechnology Principles and Processes of Biology – Class 12

1 Answer

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Best answer
Correct option is (c) 42°C

For explanation: 42°C-45°C is the ideal temperature for heat-shock treatment. Mostly calcium chloride is used in this. It is suitable for the transformation of bacterial cells such as E.coli.

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