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What may complicate the process of gene cloning within the cell?

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in Biology – Class 12 by (271k points)
What may complicate the process of gene cloning within the cell?

(a) One recognition site

(b) Foreign DNA

(c) More than one recognition site

(d) Antibody

This question was posed to me in an international level competition.

This intriguing question originated from Tools of Recombinant DNA Technology: Cloning Vectors in chapter Biotechnology Principles and Processes of Biology – Class 12

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Right answer is (c) More than one recognition site

The best explanation: Recognition site is the sequence of DNA where the DNA is cleaved. The presence of more than one recognition site will lead to several cuts within DNA leading to fragmentation of DNA. This will complicate the process of gene cloning.

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