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Competent cells can be prepared using ________

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in Biology – Class 12 by (271k points)
Competent cells can be prepared using ________

(a) electrophoresis

(b) chromatography

(c) heat-shock treatment

(d) pipetting

This question was addressed to me in semester exam.

This question is from Tools of Recombinant DNA Technology: Competent Host topic in division Biotechnology Principles and Processes of Biology – Class 12

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Best answer
The correct choice is (c) heat-shock treatment

Easy explanation: Heat-shock treatment is a method in which the vial is incubated in ice, then treated at temperature up to 50°C and again transferred on ice. It is usually used in transferring recombinant DNA within the host (bacterial cell).

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