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What happens during the ‘elution from the column’ phase in chromatography?

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What happens during the ‘elution from the column’ phase in chromatography?

(a) Components with greatest affinity elute first

(b) Components with least affinity elute first

(c) Components elute in a random manner

(d) Components elute according to their concentration in the mixture

The question was asked during a job interview.

The origin of the question is Operation of Chromatography topic in division Chromatography of Analytical Instrumentation

1 Answer

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Correct option is (b) Components with least affinity elute first

To explain: During the elution phase, different components elute at different times. Components with least affinity elute first.

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