Question

Which of the following condition is of reverse phase chromatography?

(a) The mobile phase is non-polar and stationary phase is polar

(b) The mobile phase is polar and stationary phase is non-polar

(c) Both the mobile phase and stationary phase are organic

(d) Both the mobile phase and stationary phase are inorganic

I got this question in an international level competition.

My question is based upon Chromatography in chapter Unit Operations of Bioprocess Engineering

Answer 1

Correct answer is (b) The mobile phase is polar and stationary phase is non-polar

The best I can explain: In reversed phase chromatography, the mobile phase is polar (like water), and the stationary phase is non-polar while in normal phase chromatography, the mobile phase is non-polar (100% organic), and the stationary phase is polar.