Question

Which of the following statements about column chromatography is correct?

(a) Resolution increases as the length of the column increases

(b) Mobile phase is a porous solid material with appropriate chemical properties held in the column

(c) Stationary phase is a buffered solution that percolates through mobile phase

(d) Large proteins emerge from the column sooner than small ones

I got this question at a job interview.

I want to ask this question from Working with Proteins topic in section Amino Acids, Peptides and Proteins of Biochemistry

Answer 1

Right choice is (a) Resolution increases as the length of the column increases

The best I can explain: As the length of the column increases, the overall protein band widens as it moves through the column. Individual proteins gradually separate from each other forming bands within the broader protein and this separation increases as the length of the column increases.