Question

Which of the following statements is true about two-dimensional electrophoresis?

(a) Separates proteins of identical molecular weight, same pI but different charge

(b) Separates proteins of different molecular weight and different pI

(c) Separates proteins of identical molecular weight that differ in pI

(d) Isoelectric focusing is also termed as two-dimensional electrophoresis

I had been asked this question by my college professor while I was bunking the class.

Origin of the question is Working with Proteins in section Amino Acids, Peptides and Proteins of Biochemistry

Answer 1

The correct choice is (c) Separates proteins of identical molecular weight that differ in pI

Explanation: SDS gel electrophoresis and isoelectric focusing together make up the process of two-dimensional electrophoresis. It separates proteins of identical molecular weight that differ in pI, or proteins with similar pI values but different molecular weights.