Question

Which of the following statements is true about SDS polyacrylamide chromatography?

(a) SDS polyacrylamide gel electrophoresis separates proteins on the basis of size

(b) SDS polyacrylamide gel electrophoresis separates proteins on the basis of charge

(c) SDS binds to proteins non-covalently with a stoichiometry of around one SDS molecule per three amino acids

(d) SDS binds to proteins non-covalently with a stoichiometry of around one SDS molecule per one amino acid

I have been asked this question by my college professor while I was bunking the class.

The above asked question is from Working with Proteins topic in chapter Amino Acids, Peptides and Proteins of Biochemistry

Answer 1

Right choice is (b) SDS polyacrylamide gel electrophoresis separates proteins on the basis of charge

To explain I would say: SDS polyacrylamide gel electrophoresis separates proteins on the basis of charge or mass.

SDS binds to proteins non-covalently with a stoichiometry of around one SDS molecule per two amino acids.