Question

Iterative methods include repeatedly realigning subgroups of the sequences and then by aligning these subgroups into a local alignment of all of the sequences.

(a) True

(b) False

The question was posed to me in an online interview.

My query is from Iterative Methods of Multiple Sequence Alignment in chapter Multiple Sequence Alignment of Bioinformatics

Answer 1

Right choice is (b) False

Explanation: Subgroups are aligned into a global alignment of all of the sequences. The objective is to improve the overall alignment score, such as a sum of pairs score. Selection of these groups may be based on the ordering of the sequences on a phylogenetic tree predicted in a manner similar to that of progressive alignment, separation of one or two of the sequences from the rest, or a random selection of the groups.