Question

Which of the following is untrue regarding T-Coffee?

(a) It stands for Tree-based Consistency Objective Function for alignment Evaluation

(b) It performs progressive sequence alignments as in Clustal.

(c) The global pair wise alignment is not performed using the Clustal program.

(d) The local pair wise alignment is generated by the Lalign program, from which the top ten scored alignments are selected

This question was addressed to me by my college professor while I was bunking the class.

The doubt is from Heuristic Algorithms in chapter Multiple Sequence Alignment of Bioinformatics

Answer 1

Right choice is (c) The global pair wise alignment is not performed using the Clustal program.

The explanation: The global pair wise alignment is performed using the Clustal program. The main difference is that, in processing a query, T-Coffee performs both global and local pair wise alignment for all possible pairs involved. The collection of local and global sequence alignments is pooled to form a library. The consistency of the alignments is evaluated.