Correct answer is (b) False
For explanation: CLUSTAL uses a penalty for opening a gap in a sequence alignment and an additional penalty for extending the gap by one residue. These penalties are user-defined. Gaps found in the initial alignments remain fixed. New gaps introduced as more sequences are added also receive this same gap penalty, even when they occur within an existing gap, but the gap penalties for alignment are then modified according to the average match value in the substitution matrix, the percent identity between the sequences, and the sequence lengths.