Question

Which of the following is untrue regarding TIGR Gene Indices?

(a) It is an EST database that the similar type of clustering method from UniGene

(b) It is an EST database that uses a different clustering method from UniGene

(c) It compiles data from dbEST, GenBank mRNA and genomic DNA data, and TIGR’s own sequence databased Sequences are only clustered if they are more than 95% identical for over a fortynucleotide region in pairwise comparisons

(d) None of the mentioned

I have been asked this question in a job interview.

I need to ask this question from Sequence in portion Functional Genomics & Proteomics of Bioinformatics

Answer 1

Right option is (a) It is an EST database that the similar type of clustering method from UniGene

The best I can explain: BLAST and FASTA are used to identify sequence overlaps. In the sequence assembly stage, both TIGR Assembler and CAP3 are used to construct contigs, producing a so-called tentative consensus (TC). To prevent chimerism, transcripts are clustered only if they match fully with known genes.