Question

Which of the following is untrue regarding the classic yeast two-hybrid method?

(a) Protein–protein interaction networks of yeast and a small number of other species have been subsequently determined using this method

(b) This technique is a high throughput approach

(c) Each bait and prey construct has to be prepared individually to map interactions between all proteins

(d) It has been systematically applied to study interactions at the whole proteome level

I got this question in semester exam.

This is a very interesting question from Protein Interactions topic in division Functional Genomics & Proteomics of Bioinformatics

Answer 1

The correct answer is (b) This technique is a high throughput approach

Best explanation: This technique is essentially a low throughput approach. A major flaw in this method is that it is an indirect approach to probe protein–protein interaction and has a tendency to generate false positives (spurious interactions) and false negatives (undetected interactions). It has been estimated from proteome-wide characterizations that the rate of false positives can be as high as 50%.