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Which of the following is incorrect about ARACHNE?

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Which of the following is incorrect about ARACHNE?

(a) It accepts base calls with associated quality scores assigned by Phred as input

(b) It is a free UNIX program

(c) It is for the assembly of whole-genome shotgun reads

(d) It doesn’t involve heuristic approach

This question was addressed to me during an interview.

Asked question is from Genome Sequence Assembly in portion Genome Mapping, Assembly and Comparison of Bioinformatics

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Correct answer is (d) It doesn’t involve heuristic approach

For explanation: Its unique features include using a heuristic approach similar to FASTA to align overlapping fragments, evaluating alignments using statistical scores, correcting sequencing errors based on multiple sequence alignment, and using forward-reverse constraints. It accepts base calls with associated quality scores assigned by Phred as input and produces scaffolds or a fully assembled genome.

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