Question

Which of the following is untrue about base calling and assembly programs?

(a) The first step toward genome assembly includes derive base calls

(b) The first step toward genome assembly includes assigning associated quality scores

(c) One of the steps is to assemble the sequence reads into contiguous sequences

(d) There is no identifying overlap between sequence fragments

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Asked question is from Genome Sequence Assembly topic in section Genome Mapping, Assembly and Comparison of Bioinformatics

Answer 1

Right option is (d) There is no identifying overlap between sequence fragments

The explanation: One of the steps includes identifying overlaps between sequence fragments, assigning the order of the fragments and deriving a consensus of an overall sequence. Assembling all shotgun fragments into a full genome is a computationally very challenging step. There are a variety of programs available for processing the raw sequence data.