Question

The concept of “induced fit” refers to the fact that _______

(a) When a substrate binds to an enzyme, the enzyme induces a loss of water (desolvation) from the substrate

(b) Substrate binding may induce a conformational change in the enzyme, which then brings catalytic groups into proper orientation

(c) Enzyme-substrate binding induces an increase in the reaction entropy, thereby catalyzing the reaction

(d) Enzyme specificity is induced by enzyme-substrate binding

This question was addressed to me in an interview for internship.

This is a very interesting question from Determining Enzyme Kinetic Constants from Batch Data topic in portion Homogenous Reactions of Bioprocess Engineering

Answer 1

Correct answer is (b) Substrate binding may induce a conformational change in the enzyme, which then brings catalytic groups into proper orientation

Explanation: Induced-fit model A proposed mechanism of interaction between an enzyme and a substrate. It postulates that exposure of an enzyme to a substrate causes the active site of the enzyme to change shape in order to allow the enzyme and substrate to bind.