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How many proteins were still intact after the treatment of ribosome with SDS and proteinase K?

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How many proteins were still intact after the treatment of ribosome with SDS and proteinase K?

(a) 4

(b) 8

(c) 12

(d) 16

The question was asked during an internship interview.

This is a very interesting question from Ribosome as Ribozyme topic in chapter Gene Expression : Translation of Cytogenetics

1 Answer

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Right choice is (b) 8

To explain: Even after such harsh treatment with SDS and proteinase K, 8 proteins were still retained in the ribosome. If they are removed by phenol treatment the peptidyl transfer activity is lost as they support some tertiary or quaternary structure.

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