Question

What was the unnatural experimental condition which proved to be a criticism in Robert Traut and Munro experiment?

(a) Use of low Mg^2+ concentration

(b) Use of 33% methanol

(c) Use of purified ribosome

(d) Use of puromycin

This question was posed to me in unit test.

My question is from Ribosome as Ribozyme topic in division Gene Expression : Translation of Cytogenetics

Answer 1

Right option is (a) Use of low Mg^2+ concentration

The best I can explain: The condition of 33% methanol is not usual for a celluar interior. Thus Robert Traut and Munro’s using 33% methanol (or ethanol) to carry out peptidyl transfer activity was criticized.