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Which experimental method can you use to detect 3’ end processing?

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Which experimental method can you use to detect 3’ end processing?

(a) CHIP

(b) Affinity chromatography

(c) Northern blot

(d) Immunofluorescence

This question was addressed to me at a job interview.

Enquiry is from Post-Transcriptional Modification in chapter Gene Expression : Transcription Prokaryotes of Cytogenetics

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Correct option is (b) Affinity chromatography

Easy explanation: An affinity chromatography using oligo T sephadex beads in the column can be used to detect proper 3’ processing. If the RNA is properly processed the poly A tail will adhere to the oligo T and will not come out of the column without elution.

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