Question

In an experiment you use DNA pol  I – Klenow fragment. When all other requisites for replication are added, then what will be the effect on the newly replicated DNA? Consider leading strand only.

(a) No difference from intact DNA pol I replication

(b) Replication will be slower

(c) Replication will be error prone

(d) DNA produced will be shorter

This question was addressed to me in an interview for internship.

I want to ask this question from DNA Replication Prokaryotes in division DNA Replication of Cytogenetics

Answer 1

The correct option is (d) DNA produced will be shorter

For explanation: Klenow fragment provides the 5’ -> 3’ exonuclease activity used to remove the primer. In its absence the primer remains and hence DNA produced is shorter (i.e. original length-length of primer).