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You take a circular ssDNA and to it you attach a small labeled complimentary fragment. You add different reagents and try to get free labeled probe. Which of this reagent will give you your desired result?

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You take a circular ssDNA and to it you attach a small labeled complimentary fragment. You add different reagents and try to get free labeled probe. Which of this reagent will give you your desired result?

(a) Dna b

(b) Dna c

(c) Dna G

(d) Dna a

I have been asked this question during an online interview.

My question comes from DNA Replication Prokaryotes topic in chapter DNA Replication of Cytogenetics

1 Answer

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Correct choice is (a) Dna b

For explanation I would say: Dna b has helicase activity which can separate two complementary DNA strands, this will break the link between labeled fragment and circular DNA rendering it free. Dna G mainly has primase activity while Dna a and Dna c are required for replication initiation which don’t give the desired result.

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