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You isolate the nucleolus from the nucleus and performed a density gradient centrifugation with the extract. Then you check the different for the RNA polymerase activity of the different segment. Which RNA polymerase would you expect to find in abundance?

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You isolate the nucleolus from the nucleus and performed a density gradient centrifugation with the extract. Then you check the different for the RNA polymerase activity of the different segment. Which RNA polymerase would you expect to find in abundance?

(a) RNA pol I

(b) RNA pol II

(c) RNA pol III

(d) RNA pol IV

This question was posed to me in homework.

My question is taken from Transcription in Eukaryotes : RNA Polymerases in chapter Gene Expression : Transcription Prokaryotes of Cytogenetics

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Correct choice is (a) RNA pol I

The explanation: Nucleolus is the centre for rRNA production. As RNA pol I is mainly concerned with rRNA transcription we will expect to find this in abundance. Cytosolic extract on the other hand will be abundant in RNA pol II and III.

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