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In an experiment you add growing concentration of the alpha amanitin. What will be the sequence in which RNA polymerases are affected if at all?

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In an experiment you add growing concentration of the alpha amanitin. What will be the sequence in which RNA polymerases are affected if at all?

(a) Pol I -> pol III , pol II unaffected

(b) pol I->pol I ->pol III

(c) pol III->pol II -> pol I

(d) pol II -> pol III , pol I unaffected

I had been asked this question during an online exam.

Question is from Transcription in Eukaryotes : RNA Polymerases topic in portion Gene Expression : Transcription Prokaryotes of Cytogenetics

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Right answer is (d) pol II -> pol III , pol I unaffected

Easiest explanation: RNA polymerase II is inactivated by very small level of alpha amanitin as low as 1 microgram per ml while on the other hand pol III is affected at 10 micrograms per ml concentration. Polymerase I remain unaffected even at very high alpha amanitin concentration.

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