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Type I restriction endonuclease cuts________________

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Type I restriction endonuclease cuts________________

(a) Within the recognition sequence

(b) On either side of the recognition sequence

(c) 25 to 30 bp away from the recognition sequence

(d) 1000bp away from the recognition sequence

I got this question during an online exam.

Enquiry is from Restriction Endonuclease in chapter Genomics : The Mapping and Sequencing of Genomes of Cytogenetics

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Correct answer is (d) 1000bp away from the recognition sequence

To explain: Type I restriction endonucleases are of very less practical utility, this is because although they recognize a particular sequence, they cut the DNA up to 1000bp away not providing much specificity.

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