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A methylated recognition sequence can act as a competitive inhibitor for the restriction endonuclease.

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A methylated recognition sequence can act as a competitive inhibitor for the restriction endonuclease.

(a) True

(b) False

I had been asked this question in a job interview.

My question comes from Restriction Endonuclease topic in division Genomics : The Mapping and Sequencing of Genomes of Cytogenetics

1 Answer

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Best answer
Correct choice is (a) True

Explanation: The methylated bases can bind to the enzyme but it will not be cut by the enzyme. Thus it can effectively as a competitive inhibitor as it competes with the non-methylated DNA for the same binding site.

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