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The efficiency of bead transfection is depended on the concentration of DNA in the solution.

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The efficiency of bead transfection is depended on the concentration of DNA in the solution.

(a) True

(b) False

The question was posed to me in an interview for internship.

Asked question is from Genetic Recombination topic in division Genetic Recombination of Drug Biotechnology

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Right option is (a) True

The explanation: The efficiency of this rapid technique depends on the Concentration of DNA in a solution, the timing of the addition of DNA, size and condition of the beads and the buffers utilized. In this method, the adherent cells are incubated for a brief period with glass beads in a solution containing the DNA. Then the m=solution is mixed such that the beads collide and the cells coming in between the beads may get pores.

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