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The cell fusion happens between spleen cells and _____________

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The cell fusion happens between spleen cells and _____________

(a) Red blood cells

(b) Cancer cell

(c) Myeloma cells

(d) Leukocytes

This question was posed to me during an interview for a job.

Question is from Genetic Recombination in division Genetic Recombination of Drug Biotechnology

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The correct answer is (c) Myeloma cells

The best explanation: The fusion of the cells is done between spleen cells and myeloma cells. The fusion should be done on a PEG medium. The cell plating should be done in HAT medium so that only hybridoma cells are alive after the required time. The scanning of the viable cells can be done by flow cytometry.

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