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What do you add to Myeloma cells to receive HGPRT- myeloma cells?

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What do you add to Myeloma cells to receive HGPRT- myeloma cells?

(a) 8 – Azaguanine

(b) Azaguanine

(c) Nitrogen flush

(d) Carbon dioxide flush

I got this question in final exam.

I'd like to ask this question from Genetic Recombination topic in section Genetic Recombination of Drug Biotechnology

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Right option is (a) 8 – Azaguanine

Best explanation: The myeloma cells harvested from the antigen injected mouse has to be added with 8-Azaguanine. 8-Azaguanine tends to stop the de novo pathway of and the cells which cannot survive will die. Myeloma cells which are HGPRT- will be the only cells growing in the culture due to the presence of the compound. These cells will have high viability and rapid growth.

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