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What do we get when we fuse Spleen cells and Myeloma cells?

(a) Hybridoma cells

(b) Red blood cells

(c) Killer cells

(d) Cancer myeloma B-cell

The question was asked in class test.

The query is from Genetic Recombination topic in chapter Genetic Recombination of Drug Biotechnology

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Right answer is (a) Hybridoma cells

The best I can explain: Spleen cells are fused with myeloma cells to get hybridoma cells. The fusion has to be done in PEG. The growth medium has to be feeder cells. Plating of the cells should be in HAT medium. Through ELISA we can determine the growth.

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