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Which of them is used to introduce negative supercoiling in DNA?

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Which of them is used to introduce negative supercoiling in DNA?

(a) Type 1 topoisomerase

(b) Ethidium bromide

(c) Gyrase/ type 2 topoisomerase

(d) SYBR gold

I had been asked this question in unit test.

I'd like to ask this question from Structure of dsDNA in section Biomolecules and Catalysis of Life Sciences

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The correct choice is (c) Gyrase/ type 2 topoisomerase

Easy explanation: Type 1 topoisomerase break only one strand of DNA while gyrase works on both the strands, it breaks and reseal both DNA strands and introduce negative supercoils. Ethidium bromide and SYBR gold are used to stain DNA molecules.

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