Question

Which of the following activity is not considered in Protein Fractionation which is routinely used in proteomic research?

(a) Reduce the size of the protein pool to be analyzed

(b) Remove highly expressed proteins

(c) Bring Low abundant proteins into dynamic range

(d) Bring High abundant proteins into dynamic range

The question was asked during an interview.

My enquiry is from Steps in Bioprocess Development : A Typical New Product from Recombinant DNA topic in chapter Bioprocess Development of Bioprocess Engineering

Answer 1

Correct answer is (d) Bring High abundant proteins into dynamic range

Easy explanation: The low abundant proteins are preferred over high abundant proteins as the low abundant proteins prove to be informative biomarkers and enrichment of low abundant proteins has the great advantage of obtaining much larger amount of material that can be used for further fractionations and analyses.