Question

Which of the following plot has the difficulty of extrapolating vmax?

(a) Line-weaver burk plot

(b) Eadie-Hofstee plot

(c) Michaelis-menten plot

(d) Langmuir plot

The question was asked in an internship interview.

This interesting question is from Determining Enzyme Kinetic Constants from Batch Data in chapter Homogenous Reactions of Bioprocess Engineering

Answer 1

Correct option is (c) Michaelis-menten plot

Easiest explanation: This simple procedure involves plotting (v, s) values directly vmax is the rate as s→∞ and Km is the value of s at v = vmax/2. The accuracy of this method is usually poor because of the difficulty of extrapolating to vmax.