Question

Which of the following is the correct order of sequencing?

(a) Cleaving, sequencing and ordering

(b) Sequencing, ordering and cleaving

(c) Ordering, cleaving and sequencing

(d) Ordering, sequencing and cleaving

This question was posed to me in an international level competition.

The origin of the question is Protein Sequences and Evolution in chapter Amino Acids, Peptides and Proteins of Biochemistry

Answer 1

Right option is (a) Cleaving, sequencing and ordering

To elaborate: First fragmenting of a peptide is done followed by sequencing and ordering the peptide fragments.