Question

Which of the following statements is true about ion-exchange chromatography?

(a) It separates proteins according to their size

(b) The column matrix with bound anionic groups is called cationic exchanger

(c) The column matrix with bound anionic groups is called anionic exchanger

(d) The column matrix with bound cationic groups is called cationic exchanger

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Question is from Working with Proteins topic in division Amino Acids, Peptides and Proteins of Biochemistry

Answer 1

The correct choice is (b) The column matrix with bound anionic groups is called cationic exchanger

Explanation: It involves the attachment to the column matrix of ionic groups known that bind to the anted protein.

Cationic exchanger exchanges cations with anionic groups.