Question

If one is looking for protein homologs encoded in newly sequenced genomes, one may use TBLASTN, which translates nucleotide database sequences in all six open reading frames.

(a) True

(b) False

I have been asked this question in an interview.

My doubt stems from Basic Local Alignment Search Tool (BLAST) topic in section Database Similarity Searching of Bioinformatics

Answer 1

Correct choice is (a) True

To explain I would say: This may help to identify protein coding genes that have not yet been annotated. If a DNA sequence is to be used as the query, a protein-level comparison can be done with TBLASTX. However, both programs are very computationally intensive and the search process can be very slow.